Device and method for separation of cow milk antibody by one-step membrane filtration

The invention provides a device and method for separation of a cow milk antibody by one-step membrane filtration. Specifically, the method includes: step 1: adding 2% by volume of medicinal grade diatomite into cow milk; step 2: pumping the evenly mixed cow milk into a filter by a peristaltic pump,...

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Bibliographische Detailangaben
Hauptverfasser: FU GUANGYU, LU QINGYUE, ZHANG XIAOLIANG, WU XUEWEI, LI GUILIN, ZHAO QIAOHUI, ZHENG YANLI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a device and method for separation of a cow milk antibody by one-step membrane filtration. Specifically, the method includes: step 1: adding 2% by volume of medicinal grade diatomite into cow milk; step 2: pumping the evenly mixed cow milk into a filter by a peristaltic pump, firstly passing the cow milk through a ceramic membrane to separate lipid out of the cow milk; and step 3: filtering the remaining part by a deep filtration membrane. The technical scheme integrates the deep filtration and chromatography technologies of the traditional biopharmacy industry to form aclosed system conforming to the GMP pharmaceutical standard, the ceramic membrane can separate lipid components from the cow milk, at the same time can adsorb large particulate matters by diatomite filtering, and then the obtained clarified whey can be subjected to next step chromatography, the time is 1/2 that of traditional process firstly carrying out filtration and then performing chromatography, and the economic dir