Early-stage cancer screening kit based on blood plasma exosome GRP78 mRNA

The invention discloses an early-stage cancer screening kit based on blood plasma exosome GRP78 mRNA. The early-stage cancer screening kit comprises a pair of exosome GRP78 mRNA fluorogenic quantitative PCR primers, and the nucleotide sequences of the primers are shown in SEQ ID NO:1-2. By adopting the kit, early-stage screening of multiple types of cancer can be completed with only 5ml of blood,and an early-stage cancer screening method is good in specificity, high in sensitivity, high in efficiency, good in practicability, good in noninvasiveness and high in reliability, and easy in clinical popularization in medical institutes of different grades, is particularly applicable to physical screening and early-stage warming of susceptible tumor patients, and has great significant values. 本发明公开了种基于血浆外泌体中GRP78 mRNA的癌症早期筛查试剂盒，含有对外泌体GRP78 mRNA荧光定量PCR引物，引物的核苷酸序列如SEQ ID NO：1～2所示。本发明可以仅仅利用5ml血液就能完成多种癌症的早期筛查，使得癌症的早期检测方法特异好、敏感、高效、实用，无创，可靠度高，易于各级医疗机构临床推广，也特别适用于肿瘤易感患者的体检筛查和早期预警，具有重要的意义价值。