Method for quickly identifying normal differentiation and abnormal differentiation of retinal pigment epithelium

The invention discloses a method for quickly identifying normal differentiation and abnormal differentiation of retinal pigment epithelium (RPE). The method comprises the steps of applying a cell lysis buffer for extracting total RNA (Ribonucleic Acid) in the retinal pigment epithelium, carrying out...

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Bibliographische Detailangaben
Hauptverfasser:	ZHANG ZHIWEI, FAN GUOPING, FANG PANFENG, MU JINGHAI, LI NING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a method for quickly identifying normal differentiation and abnormal differentiation of retinal pigment epithelium (RPE). The method comprises the steps of applying a cell lysis buffer for extracting total RNA (Ribonucleic Acid) in the retinal pigment epithelium, carrying out in-vitro reverse transcription synthesis and applying a real-time fluorescence quantification PCR (Polymerase Chain Reaction) instrument for quantitative determination. The invention provides a new method for real-time detecting a differentiation state of the RPE during a culture process. 本发明公开了种快速鉴定视网膜色素上皮细胞(RPE)正常分化和异常分化的方法，涉及了运用细胞裂解液提取视网膜色素上皮细胞内的总RNA，并进行体外逆转录合成以及运用实时荧光定量PCR仪进行定量检测。本发明为实时检测RPE细胞在培养过程中的分化状态提供了新方法。