Tissue culture seedling raising method for hybrid seeds of paphiopedilum barbigerum flowers and paphiopedilum villosum

The invention discloses a tissue culture seedling raising method for hybrid seeds of paphiopedilum barbigerum flowers and paphiopedilum villosum. The method comprises steps as follows: (1), crossbreeding; (2), disinfection treatment of the hybrid seeds; (3), germination culture of the hybrid seeds: a culture medium is prepared from saccharose, potatoes, coconut milk, activated carbon and agar powder; (4), protocorm culture of the hybrid seeds: a culture medium is prepared from saccharose, the potatoes, bananas, activated carbon and the agar powder; (5), rooting culture of the hybrid seeds: a culture medium is prepared from saccharose, the potatoes, the bananas, activated carbon and the agar powder, PH is 5.5, illumination culture is performed for 12 hr/d, illumination is 1500-1200 LUX, andculture temperature is 25 DEG C plus or minus 1 DEG C; (6), transplanting of test-tube plantlets. According to the method, components and proportional parameters of the culture media at all stages oftissue culture seedling r