Method for preparing sotolon on basis of column chromatography separation and application to cigarettes

The invention provides a separation preparation method of sotolon and an application to cigarettes. Fenugreek seeds taken as the raw material are subjected to baking, extraction and concentration, a fenugreek tincture or extract is obtained, an organic solvent is taken as an eluent system, and the sotolon is obtained through silica-gel column chromatography separation. GC-MS detection indicates that GC purity of the sotolon is higher than 99%. The method is simple to operate, low in equipment requirement and shorter in consumed time. 本发明提供了种葫芦巴内酯分离制备方法及在卷烟中应用，以葫芦巴籽为原料，葫芦巴籽经烘焙、萃取、浓缩得到葫芦巴酊剂或浸膏，以有机溶剂为洗脱剂体系，利用硅胶柱层析分离得到葫芦巴内酯。经GC-MS检测葫芦巴内酯GC纯度>99％。本发明所述的方法操作简单，设备要求低，耗时较少。