Improved separation method for cell body and extension of liver cancer transfer cells

The invention provides an improved separation method for a cell body and an extension of liver cancer transfer cells. According to the invention, a Boyden chamber is used for separating the cell bodyand the extension of the liver cancer transfer cells, from a subcellular structure, the improved Boyden chamber is used for separating the cell body and the extension of the liver cancer transfer cells, and the technologies such as Western-blot, RNA dyeing and immunofluorescence are used for verifying the separating results. The method can be used for researching a differential expression condition in the cell body and the extension of a HCCLM 3 liver cancer cell and screening minimal RNA related to HCC infiltration and metastasis processes, and the method provides novel thinking for subsequent researches and related clinical diagnosis and treatment for a HCC tumor biomarker. 种改良的肝癌转移细胞胞体和突出的分离方法，利用Boyden小室分离肝癌转移细胞的突出与胞体，从亚细胞结构出发，运用改良的Boyden小室法分离肝癌转移细胞胞体和突出，利用Western-blot、RNA染色、免疫荧光等技术验证分离结果。可于研究在HCCLM3 肝癌细胞胞体与突出中