RAPID CONCENTRATION, RECOVERY AND DETECTION OF PATHOGENS IN FOOD SAMPLES

Methods for rapidly concentrating a food sample for efficient detection of bacteria are disclosed. A microfiltration approach followed by centrifugation was used to concentrate the cells with an enzyme (e.g., a protease) added at the beginning of the process to facilitate more efficient micro-filter...

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Hauptverfasser:	KREKE THOMAS RICHARD, XIMINES EDUARDO DE AQUINO, LADISCH MICHAEL RALPH, DEERING AMANDA, LIU XINGYA (LINDA), HARDENSTEIN JAYCEY, TUNGARE ALISHA, FOSTER KIRK SOLON, KU SEOCKMO, JONES JAMES THOMAS
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Methods for rapidly concentrating a food sample for efficient detection of bacteria are disclosed. A microfiltration approach followed by centrifugation was used to concentrate the cells with an enzyme (e.g., a protease) added at the beginning of the process to facilitate more efficient micro-filtering. The enzyme was found to have no significant effect on cell viability. 披露了用于快速浓缩食物样品以有效检测细菌的方法。使用微过滤方法，然后离心来浓缩细胞，其中在该方法开始时添加酶(例如蛋白酶)以促进更有效的微过滤。发现该酶对细胞活力没有显著的影响。