Proteases with modified propeptide regions

The present invention provides methods and compositions for the production of mature proteases in bacterial host cells. The compositions include polynucleotides encoding serine protease sequences withmodified or heterologous propeptide regions; polypeptides comprising serine proteases with modified or heterologous propeptide regions; expression cassettes, DNA constructs, vectors, and chromosomescomprises such polynucleotides; and bacterial host cells comprising such polynucleotides. The methods include methods for enhancing the production of mature proteases in bacterial host cells (e.g. Bacillus sp. host cells). The produced proteases find use in the industrial production of enzymes, suitable for use in various industries, including but not limited to the cleaning, animal feed and textile processing industry. 本发明提供了用于在细菌宿主细胞中产生成熟蛋白酶的方法和组合物。这些组合物包括编码具有经修饰的或异源的前肽区域的丝氨酸蛋白酶序列的多核苷酸；包含具有经修饰的或异源的前肽区域的丝氨酸蛋白酶的多肽；包含这样的多核苷酸的表达盒、DNA构建体、载体和染色体；以及包含这样的多核苷酸的细菌宿主细胞。这些方法包括用于增强细菌宿主细胞(例如，芽孢杆菌属物种宿主细胞)中成熟蛋白酶产生的方法。所产生的蛋白酶可用于适用于不同