Method for extracting free DNA from serum and plasma

The invention belongs to the technical field of molecular biology, and particularly relates to a method for extracting free DNA from serum and plasma. The method for extracting free DNA from serum andplasma comprises the following steps: cracking a sample to be tested by adopting a specific cracking...

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Hauptverfasser: WANG MEIJIN, AI PENG, TAO YONG, HUANG YUPING, LU MANYUN, LIU TENGFEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of molecular biology, and particularly relates to a method for extracting free DNA from serum and plasma. The method for extracting free DNA from serum andplasma comprises the following steps: cracking a sample to be tested by adopting a specific cracking solution; and then enriching and purifying free DNA by adopting a self-made adsorption column membrane, thereby obtaining high-quality free DNA. The free DNA extracted withthe method for extracting the free DNA from serum and plasma has the advantages of high yield and high purity; and meanwhile,the method for extracting the free DNA from serum and plasma, provided by the invention, can also prevent free radicals from damaging the free DNA, ensures the integrity of the extracted free DNA, isbeneficial to subsequent prenatal diagnosis and early tumor screening, and has great significance to clinical medicine. 本发明属于分子生物学技术领域,具体涉及种从血清血浆中提取游离DNA的方法。本发明提供的从血清血浆中提取游离DNA的方法是:采用特定的裂解液对待测样本进行裂解,然后再采用自制的吸附柱膜对游离的DNA进行富集纯化,从而