Method used for capturing interaction sites among chromatin short segments

The invention belongs to the technical field of gene engineering, and more specifically relates to a method used for rapid capturing of interaction sites among chromatin short segments. According to the method, HeLa S3 cell line is taken as an analysis object; the method comprises following steps: cell cycle synchronization culture; cell nucleus extraction; sequencing library preparation; sequencing library amplification; sequencing comparison; and analysis and positioning. According to the method, chromosome conformation capture is adopted, so that the interaction segments among specific chromatins can be observed preferably, the specific interaction effect is capable of proving expression regulating analysis of gene with foundation, and providing chromatin three dimensional structure analysis with foundation. The specificity of the method is verified preferably; and it is promising to obtain better results in analysis on the interaction among chromatin short segments. 本申请属于基因工程技术领域，具体涉及种快速捕获染色质短片段之间相互作用位点的方