Method of extracting total RNA from leaves and roots of corn seedlings

The invention discloses a method of extracting total RNA from leaves and roots of corn seedlings. The extraction method comprises the following steps: facility preprocessing, seedling selection, seedling preprocessing, dissociation of nucleoprotein compounds, RNA precipitation, RNA washing, RNA dissolution, and RNA storage. The extraction method is simple and is easy to perform, the quality of extracted RNA is high, and the method can be used to extract the total RNA of corn roots and leaves. 本发明公开了种玉米幼苗叶片及根的总RNA的提取方法，经过设备的预处理、幼苗的选择、幼苗的预处理、核蛋白复合物的解离、RNA的沉淀、RNA的洗涤、RNA的溶解和存储步骤，完成了玉米幼苗叶片及根的总RNA的提取方法；本发明的优点在于：简便易行、提取的RNA质量高、可同时适用于于玉米根部及叶片的RNA提取。