Method for detecting neomycin sulfate content in triamcinolone acetonide acetat

The invention provides a method for detecting detecting neomycin sulfate content in triamcinolone acetonide acetat. The method comprises the following steps: preparing bacterium suspension; preparinga standard solution; preparing a test solution; preparing a negative sample solution; culturing; detecting. The method is high in specificity and repeatability; compared with a liquid chromatography,the method has the advantages of being simple to operate, low in test cost, and convenient to popularize and apply; dichloromethane is treated as a solvent, so that the solubleness is high, and creamsubstrate can be quickly dissolved in dichloromethane; the influence of the cream substrate is greatly avoided; the detecting neomycin sulfate content detecting result is high in reliability and highin accuracy degree. 本发明提供了种曲咪新乳膏中硫酸新霉素含量的测定方法，包括如下步骤：菌悬液的制备、标准溶液的制备、供试品溶液的制备、阴性样品溶液的制备、培养与测定。本发明所述的种曲咪新乳膏中硫酸新霉素含量的测定方法，具有良好的专属性和重复性，与液相色谱法相比，操作简单、实验成本低，便于推广和应用，二氯甲烷作为溶剂的溶解性强，乳膏基质会快速溶解至二氯甲烷中，排除了大部分乳膏基质的影响，硫酸新霉素含量测定结果可靠性强、准确度高。