High-temperature-resistant high-specific-activity bacterial phytase gene suitable for expression of methylotrophic yeast

The invention discloses an acquisition method of a high-temperature-resistant high-specific-activity bacterial phytase gene suitable for high-efficiency secretory expression in pichia pastoris. The method comprises the following steps: firstly optimizing and modifying 9 phytase genes from yersinia into genes suitable for the expression of pichia pastoris, then performing the in-vitro modificationfor the gene family, establishing a phytase gene mutant library, performing saccharomyces cerevisiae library expression and high-throughput screening, obtaining a high-temperature-resistant phytase gene YAPPA102, establishing the high-temperature-resistant phytase gene YAPPA102 onto a pichia pastoris expression carrier, thereby realizing the high-efficiency expression of the phytase. The expressedphytase has high temperature resistance and is stable in structure. 本发明公开了种能够在毕赤酵母中高效分泌表达的耐高温高比活植酸酶基因获取方法，具体过程是：将来自耶尔森菌属的9个植酸酶基因先优化改造成适合毕赤酵母表达的基因，然后经过基因家族体外改造，构建植酸酶基因突变体库，通过酿酒酵母文库表达和高通量筛选，获得耐高温的植酸酶基因YAPPA102，将耐高温植酸酶基因YAPPA102