Disulfide bond combining heat-resistant mutant lipase as well as preparation method and application thereof

The invention discloses disulfide bond combining heat-resistant mutant lipase as well as a preparation method and application thereof. The lipase is 2N mutant lipase shown as the amino acid sequence SEQ ID NO.1, 2S mutant lipase shown as the amino acid sequence SEQ ID NO.2, 3N mutant lipase shown as the amino acid sequence SEQ ID NO.3, and 3S mutant lipase shown as the amino acid sequence SEQ ID NO.4. S8-214 lipase is used as a parent; 1 pair of disulfide bonds with the weak effect improving effect is introduced in one or two different located regions/between the located regions of the disulfide bonds S8-214; or one pair or two pairs of disulfide bonds with the strong effect improving effect are simultaneously introduced into the identical located region of the disulfide bonds S8-214; thecombination optimization of the disulfide bond mutation is realized, wherein the disulfide bond combination effect in/between different regions is better. The obtained mutant lipase has high thermal stability, and is particul