High temperature-resistant Saccharomyces cerevisiae strain and constructing method thereof

The invention discloses a screening and constructing method for high temperature-resistant Saccharomyces cerevisiae. The method comprises the following steps: carrying out ARTP plasma mutagenesis on astrain AY12-G used as an original strain, carrying out primary screening at 37 DEG C to obtain 150 s...

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Bibliographische Detailangaben
Hauptverfasser:	HAO AILI, DONG JIAN, XIAO DONGGUANG, FU XIAOMENG, GUO XUEWU, ZHANG CUIYING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a screening and constructing method for high temperature-resistant Saccharomyces cerevisiae. The method comprises the following steps: carrying out ARTP plasma mutagenesis on astrain AY12-G used as an original strain, carrying out primary screening at 37 DEG C to obtain 150 strains, carrying out genome rearrangement on the 150 strains, and primarily screening the 150 strains at 37 DEG C to obtain 137 strains; and carrying out 35 DEG C corn hydrolysate fermentation on the 137 strains to obtain 14 strains, carrying out 35 DEG C simultaneous saccharification and fermentation on the 14 strains and 48 h cell survival rate determination to obtain 7 strains having improved high temperature fermentation performances and 48 h cell survival rate, and carrying out 41 DEG C and 42 DEG C high temperature domestication on the 7 strains to finally obtain a strain X-130 having excellent high temperature fermentation performances and significantly improved 48 h cell survival rate. Compared with the ori