Method of preparing goat milk active drink by using lactobacillus rhamnosus protease
The present invention discloses a method of preparing goat milk active drink by using lactobacillus rhamnosus protease. A lactobacillus rhamnosus culture medium is inoculated with lactobacillus rhamnosus in an inoculation amount, culturing is carried out, the cultured lactobacillus rhamnosus is cent...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The present invention discloses a method of preparing goat milk active drink by using lactobacillus rhamnosus protease. A lactobacillus rhamnosus culture medium is inoculated with lactobacillus rhamnosus in an inoculation amount, culturing is carried out, the cultured lactobacillus rhamnosus is centrifuged, a centrifuged lactobacillus rhamnosus precipitate is collected, then the lactobacillus rhamnosus precipitate is washed using a sterile normal saline, and the washed lactobacillus rhamnosus is centrifuged to obtain lactobacillus rhamnosus paste; a phosphate buffer is added into the lactobacillus rhamnosus paste, the mixture is centrifuged to obtain a cell wall protease enzyme solution, and the cell wall protease enzyme solution is freeze-dried to obtain lactobacillus rhamnosus cell wallprotease bacterial powder; and the lactobacillus rhamnosus cell wall protease bacterial powder and CaCl2 are added into degreased fresh goat milk, enzymolysis is carried out, then beta-cyclodextrin, honey raisin tree fruit po |
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