Molecular marking method for identifying fertility of single plant in male sterile dual-purpose line of chili during seedling stage

The invention discloses a molecular marking method for identifying fertility of a single plant in a male sterile dual-purpose line of chili during seedling stage. The method includes the steps of: firstly, artificially designing a pair of specific nucleotide sequence as a primer, and performing PCR amplification to genome DNA of every single plant in the male sterile dual-purpose line of chili with the primer; performing electrophoresis to the amplification products with 0.8% sepharose gel, if a specific band appears in the electrophoresis result, it means that the amplification product in this electrophoresis lane comes from a stamen fertile plant, or otherwise the amplification product in this electrophoresis lane comes from a male sterile plant. The method has simple and quick processesand is stable and reliable. The identification result is coincided with identification result of fertility with floral organs in field. The method has good repeatability and is very suitable for molecular marking assisted br