Method for developing bidens bipinnata plant SSR (Simple Sequence Repeats) primer based on transcriptome sequencing

The invention discloses a method for developing a bidens bipinnata plant SSR (Simple Sequence Repeats) primer based on transcriptome sequencing. The method comprises the following steps: (1) acquiringtranscriptome data; (2) identifying an SSR primer; and (3) designing the SSR primer. 28 pairs of SSR...

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Hauptverfasser:	JIN HAITAO, PANG HONGBO, WU LONGKUN, LI YUEYING, GAO QIU, LIU HONGXIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CALCULATING CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF COMPUTING CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES COUNTING CULTURE MEDIA ELECTRIC DIGITAL DATA PROCESSING ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a method for developing a bidens bipinnata plant SSR (Simple Sequence Repeats) primer based on transcriptome sequencing. The method comprises the following steps: (1) acquiringtranscriptome data; (2) identifying an SSR primer; and (3) designing the SSR primer. 28 pairs of SSR primers of the invention are obtained by screening 6 species of bidens bipinnata and 9 different geographical region populations in the bidens bipinnata species, the primer has polymorphisms in 15 bidens bipinnata species, the 28 primer pairs provided by the invention can be directly applied to more species of the bidens bipinnata to analyze germplasm resources and genetic diversity, and a good foundation can be set for the molecular marker assistant breeding of the bidens bipinnata. 本发明公开种基于转录组测序开发鬼针草植物SSR引物的方法，包括如下步骤：(1)转录组数据的获得；(2)SSR引物的识别(3)SSR引物的设计；本发明的28对SSR引物是通过来源于鬼针草属6个种和鬼针草种中9个不同地理区域居群筛选得到，在15个鬼针草物种中均具有多态性，可以直接将本发明所提供的28个引物对应用于更多鬼针草品种上，进行种质资源和遗传多样性分析，为鬼针草分子标记辅助育种奠定了良好的基础。