NF-kappa B dual-luciferase reporter cell capable of stably expressing pig-origin RIG-I receptor genes and construction method thereof
The invention belongs to the technical field of biology and specifically relates to an NF-kappa B dual-luciferase reporter cell system capable of stably expressing pig-origin RIG-I receptor genes anda construction method thereof. The cell system is an HEK293-NF-kappa B cell which the pig-origin RIG-...
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| creator | ZHU JIANZHONG LI SHUANGJIE CHEN NANHUA HE SHAN ZHU MEIQIN ZHOU RONGYUN |
| description | The invention belongs to the technical field of biology and specifically relates to an NF-kappa B dual-luciferase reporter cell system capable of stably expressing pig-origin RIG-I receptor genes anda construction method thereof. The cell system is an HEK293-NF-kappa B cell which the pig-origin RIG-I (pRIG-I) receptor genes are transferred into, and the HEK293-NF-kappa B cell is an HEK293 cell which NR-kappa B firefly luciferase and internal reference renilla luciferase are guided into. The stable cell system disclosed by the invention can be applied to researching detection on downstream NF-kappa B promoter reporter genes activated by pRIG-I. The invention further provides a biological material for an RNA-Seq technology to screen stable cell internal differential expression genes under silent and active states of the pRIG-I in high throughput; furthermore, a foundation and a platform are established for further researching species specificity of the pRIG-I.
本发明属于生物技术领域，具体涉及稳定表达猪源RIG-I受体基因的NF-κB双荧光素酶报告细胞系及其构建 |
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本发明属于生物技术领域，具体涉及稳定表达猪源RIG-I受体基因的NF-κB双荧光素酶报告细胞系及其构建</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2018</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20180323&DB=EPODOC&CC=CN&NR=107828732A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20180323&DB=EPODOC&CC=CN&NR=107828732A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHU JIANZHONG</creatorcontrib><creatorcontrib>LI SHUANGJIE</creatorcontrib><creatorcontrib>CHEN NANHUA</creatorcontrib><creatorcontrib>HE SHAN</creatorcontrib><creatorcontrib>ZHU MEIQIN</creatorcontrib><creatorcontrib>ZHOU RONGYUN</creatorcontrib><title>NF-kappa B dual-luciferase reporter cell capable of stably expressing pig-origin RIG-I receptor genes and construction method thereof</title><description>The invention belongs to the technical field of biology and specifically relates to an NF-kappa B dual-luciferase reporter cell system capable of stably expressing pig-origin RIG-I receptor genes anda construction method thereof. The cell system is an HEK293-NF-kappa B cell which the pig-origin RIG-I (pRIG-I) receptor genes are transferred into, and the HEK293-NF-kappa B cell is an HEK293 cell which NR-kappa B firefly luciferase and internal reference renilla luciferase are guided into. The stable cell system disclosed by the invention can be applied to researching detection on downstream NF-kappa B promoter reporter genes activated by pRIG-I. The invention further provides a biological material for an RNA-Seq technology to screen stable cell internal differential expression genes under silent and active states of the pRIG-I in high throughput; furthermore, a foundation and a platform are established for further researching species specificity of the pRIG-I.
本发明属于生物技术领域，具体涉及稳定表达猪源RIG-I受体基因的NF-κB双荧光素酶报告细胞系及其构建</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2018</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjjEOwjAQBNNQIOAPxwMsQVIkLUQE0qRA9NHhnB0LY1tnR4IH8G9S8ACq3WJ2tMvs0zXigSEgHGGY0Ao7SaOIMRIwBc-JGCRZCxID3i2BVxDT3N5Ar8AUo3EagtHCs9HGwbU9i3beSgrJM2hyFAHdANK7mHiSyXgHT0qjHyCNxOTVOlsotJE2v1xl2-Z0qy9iftBTDChnS-rrbr8rq7wqi_xQ_MN8AUtsSpQ</recordid><startdate>20180323</startdate><enddate>20180323</enddate><creator>ZHU JIANZHONG</creator><creator>LI SHUANGJIE</creator><creator>CHEN NANHUA</creator><creator>HE SHAN</creator><creator>ZHU MEIQIN</creator><creator>ZHOU RONGYUN</creator><scope>EVB</scope></search><sort><creationdate>20180323</creationdate><title>NF-kappa B dual-luciferase reporter cell capable of stably expressing pig-origin RIG-I receptor genes and construction method thereof</title><author>ZHU JIANZHONG ; LI SHUANGJIE ; CHEN NANHUA ; HE SHAN ; ZHU MEIQIN ; ZHOU RONGYUN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN107828732A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2018</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ZHU JIANZHONG</creatorcontrib><creatorcontrib>LI SHUANGJIE</creatorcontrib><creatorcontrib>CHEN NANHUA</creatorcontrib><creatorcontrib>HE SHAN</creatorcontrib><creatorcontrib>ZHU MEIQIN</creatorcontrib><creatorcontrib>ZHOU RONGYUN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ZHU JIANZHONG</au><au>LI SHUANGJIE</au><au>CHEN NANHUA</au><au>HE SHAN</au><au>ZHU MEIQIN</au><au>ZHOU RONGYUN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>NF-kappa B dual-luciferase reporter cell capable of stably expressing pig-origin RIG-I receptor genes and construction method thereof</title><date>2018-03-23</date><risdate>2018</risdate><abstract>The invention belongs to the technical field of biology and specifically relates to an NF-kappa B dual-luciferase reporter cell system capable of stably expressing pig-origin RIG-I receptor genes anda construction method thereof. The cell system is an HEK293-NF-kappa B cell which the pig-origin RIG-I (pRIG-I) receptor genes are transferred into, and the HEK293-NF-kappa B cell is an HEK293 cell which NR-kappa B firefly luciferase and internal reference renilla luciferase are guided into. The stable cell system disclosed by the invention can be applied to researching detection on downstream NF-kappa B promoter reporter genes activated by pRIG-I. The invention further provides a biological material for an RNA-Seq technology to screen stable cell internal differential expression genes under silent and active states of the pRIG-I in high throughput; furthermore, a foundation and a platform are established for further researching species specificity of the pRIG-I.
本发明属于生物技术领域，具体涉及稳定表达猪源RIG-I受体基因的NF-κB双荧光素酶报告细胞系及其构建</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | NF-kappa B dual-luciferase reporter cell capable of stably expressing pig-origin RIG-I receptor genes and construction method thereof |
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