NF-kappa B dual-luciferase reporter cell capable of stably expressing pig-origin RIG-I receptor genes and construction method thereof

The invention belongs to the technical field of biology and specifically relates to an NF-kappa B dual-luciferase reporter cell system capable of stably expressing pig-origin RIG-I receptor genes anda construction method thereof. The cell system is an HEK293-NF-kappa B cell which the pig-origin RIG-I (pRIG-I) receptor genes are transferred into, and the HEK293-NF-kappa B cell is an HEK293 cell which NR-kappa B firefly luciferase and internal reference renilla luciferase are guided into. The stable cell system disclosed by the invention can be applied to researching detection on downstream NF-kappa B promoter reporter genes activated by pRIG-I. The invention further provides a biological material for an RNA-Seq technology to screen stable cell internal differential expression genes under silent and active states of the pRIG-I in high throughput; furthermore, a foundation and a platform are established for further researching species specificity of the pRIG-I. 本发明属于生物技术领域，具体涉及稳定表达猪源RIG-I受体基因的NF-κB双荧光素酶报告细胞系及其构建