Microfluidic chip for single cell micro-droplet sample preparation

The invention relates to the technical field of microfluidic chip, and in particular, relates to a microfluidic chip for single cell micro-droplet sample preparation, wherein the microfluidic chip includes a chip body, and a single cell micro-droplet sample preparation unit and a blank droplet remov...

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Bibliographische Detailangaben
Hauptverfasser:	CHEN YONG, RUAN MEILIN, CHEN CHAOHUI, HE RONGXIANG, ZHANG ZHENGTAO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the technical field of microfluidic chip, and in particular, relates to a microfluidic chip for single cell micro-droplet sample preparation, wherein the microfluidic chip includes a chip body, and a single cell micro-droplet sample preparation unit and a blank droplet removal unit which are distributed on the chip body; the single cell micro-droplet sample preparation unit and the blank droplet removal unit are connected by a micro channel of the microfluidic chip; single cell micro-droplet sample preparation with high throughput and high proportion is achieved by thesingle cell micro-droplet sample preparation unit and the blank droplet removal unit; different cell samples are screened by controlling the size of a micro-structure of the blank droplet removal unit; therefore, 95% of micro droplets in the cell micro-droplet sample preparation are wrapped with cells, for subsequent micro-droplet based cell culture, single cell in situ fluorescence detection, single cell PCR and the lik