Genetically engineered bacterium strain for producing D-1,2,4-butantriol by utilizing D-xylose as well as construction method and application of genetically engineered bacterium
The invention discloses a genetically engineered bacterium strain for producing D-1,2,4-butantriol by utilizing D-xylose. The construction method comprises the following steps: constructing two plasmids such as PMB and PGP, introducing the two constructed plasmids into Escherichia coli E.coliBL21(DE...
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| creator | WANG XIN GAO QIAN HU SHEWEI JIANG MINGJUN CHEN KEQUAN XU NANA OUYANG PINGKAI |
| description | The invention discloses a genetically engineered bacterium strain for producing D-1,2,4-butantriol by utilizing D-xylose. The construction method comprises the following steps: constructing two plasmids such as PMB and PGP, introducing the two constructed plasmids into Escherichia coli E.coliBL21(DE3) to construct a recombination strain E.coliBL21-PMB-PGP, and performing whole-cell catalysis to produce the D-1,2,4-butantriol. According to the method disclosed by the invention, the problems that a fermentation method is long in production cycle, the metabolite is complicated, the substrate conversion rate is low, the product is difficult to separate and extract and the energy consumption is high are solved; the defect that the catalytic process is difficult to realize in an enzymatic methodis overcome; and the catalytic efficiency is improved, and the enzyme purification process is saved.
本发明公开了株利用D-木糖生产D-1,2,4-丁三醇的基因工程菌，先构建两种质粒PMB和PGP，再将已构建好的双质粒导入到大肠杆菌E.coliBL21(DE3)中构建好重组菌株E.coliBL21-PMB-PGP，全细胞催化生产D-1,2,4丁三 |
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本发明公开了株利用D-木糖生产D-1,2,4-丁三醇的基因工程菌，先构建两种质粒PMB和PGP，再将已构建好的双质粒导入到大肠杆菌E.coliBL21(DE3)中构建好重组菌株E.coliBL21-PMB-PGP，全细胞催化生产D-1,2,4丁三</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2018</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjrmOwkAQRJ1sgFj-oTfHEuZYSBHHEhGRo_G4bVpqukdzaNf81f4h5sghKJVUVXqqXvb_g4KRrGFuAaUhQfRYQWlsRE_pDCF6QwK1enBeq2RJGljnxXA8nOZlikaiJ2UoW0iRmC6P_q9lDQgmwC8y39yqdKxkI6nAGeNJKzDSyTnuDtxjraF5cegz-6gNBxw8vZ99bTeH1S5Hp0cMztgb4LjaF6P5ophNJ9_LyTubK4dCW6s</recordid><startdate>20180320</startdate><enddate>20180320</enddate><creator>WANG XIN</creator><creator>GAO QIAN</creator><creator>HU SHEWEI</creator><creator>JIANG MINGJUN</creator><creator>CHEN KEQUAN</creator><creator>XU NANA</creator><creator>OUYANG PINGKAI</creator><scope>EVB</scope></search><sort><creationdate>20180320</creationdate><title>Genetically engineered bacterium strain for producing D-1,2,4-butantriol by utilizing D-xylose as well as construction method and application of genetically engineered bacterium</title><author>WANG XIN ; GAO QIAN ; HU SHEWEI ; JIANG MINGJUN ; CHEN KEQUAN ; XU NANA ; OUYANG PINGKAI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN107815436A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2018</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>WANG XIN</creatorcontrib><creatorcontrib>GAO QIAN</creatorcontrib><creatorcontrib>HU SHEWEI</creatorcontrib><creatorcontrib>JIANG MINGJUN</creatorcontrib><creatorcontrib>CHEN KEQUAN</creatorcontrib><creatorcontrib>XU NANA</creatorcontrib><creatorcontrib>OUYANG PINGKAI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WANG XIN</au><au>GAO QIAN</au><au>HU SHEWEI</au><au>JIANG MINGJUN</au><au>CHEN KEQUAN</au><au>XU NANA</au><au>OUYANG PINGKAI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Genetically engineered bacterium strain for producing D-1,2,4-butantriol by utilizing D-xylose as well as construction method and application of genetically engineered bacterium</title><date>2018-03-20</date><risdate>2018</risdate><abstract>The invention discloses a genetically engineered bacterium strain for producing D-1,2,4-butantriol by utilizing D-xylose. The construction method comprises the following steps: constructing two plasmids such as PMB and PGP, introducing the two constructed plasmids into Escherichia coli E.coliBL21(DE3) to construct a recombination strain E.coliBL21-PMB-PGP, and performing whole-cell catalysis to produce the D-1,2,4-butantriol. According to the method disclosed by the invention, the problems that a fermentation method is long in production cycle, the metabolite is complicated, the substrate conversion rate is low, the product is difficult to separate and extract and the energy consumption is high are solved; the defect that the catalytic process is difficult to realize in an enzymatic methodis overcome; and the catalytic efficiency is improved, and the enzyme purification process is saved.
本发明公开了株利用D-木糖生产D-1,2,4-丁三醇的基因工程菌，先构建两种质粒PMB和PGP，再将已构建好的双质粒导入到大肠杆菌E.coliBL21(DE3)中构建好重组菌株E.coliBL21-PMB-PGP，全细胞催化生产D-1,2,4丁三</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Genetically engineered bacterium strain for producing D-1,2,4-butantriol by utilizing D-xylose as well as construction method and application of genetically engineered bacterium |
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