Immobilized plasminogenase composition, preparation process, use and device comprising such a composition

The invention relates to an enzymatic composition comprising: - at least one enzyme, termed plasminogenase, for converting, into plasmin, plasminogen from a blood plasma medium comprising plasminogen;- a solid support that is insoluble in aqueous solution, the solid support having dimensions suitable for being able to be retained on a filter having a cut-off threshold of less than or equal to 0.22 [mu]m; characterized in that said plasminogenase is bound to the solid support and remains bound to this support on contact with a blood plasma medium and in that the composition is in the dry state. The invention also relates to a process for preparing such an enzymatic composition, to the use thereof and to a device (20) for preparing a blood plasma medium ex vivo rich in sterile plasmin and free of enzymatic composition. 本发明涉及包含下列的酶组合物：-至少种酶，称为纤溶酶原酶，其将包含纤溶酶原的血液血浆介质中的纤溶酶原转变为纤溶酶；-在水性溶液中不可溶的固体载体，该固体载体具有适合于能保留在具有小于或等于0.22μm的截留阈值的滤器上的尺寸；其特征在于，所述纤溶酶原酶结合所述固体载体并且在与血液血浆介质相接触时保持与该载体结合，并且组合物处于干燥状态。本发明还涉及制备此类酶组合物的方法、其用途和用于制备