Fluorescence detection method for lead ions

Fluorescence detection method for lead ions

The invention discloses a fluorescence detection method for lead ions. By using the characteristic that a lead ion specificity DNA (Pb aptamer) (5'-GGGTGGGTGGGTGGGT-3') can be combined with lead ion specificity to form a G tetrahedral structure and the characteristic that a fluorescent dye...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: XU HUI, LYU JUBO, ZHANG YAHUI
Format: Patent
Sprache:chi ; eng
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator XU HUI
LYU JUBO
ZHANG YAHUI
description The invention discloses a fluorescence detection method for lead ions. By using the characteristic that a lead ion specificity DNA (Pb aptamer) (5'-GGGTGGGTGGGTGGGT-3') can be combined with lead ion specificity to form a G tetrahedral structure and the characteristic that a fluorescent dye Sybr Green I can be combined with a double-chain DNA so that the fluorescence intensity is greatly enhanced by compared with a free state, so that the lead ion specificity DNA (Pb aptamer) is combined with a partially complementary chain DNA (Pb aptamer-com), then the fluorescence intensity is greatly enhanced through the addition of the fluorescent dye Sybr Green I, and then lead ions are added to be combined with Pb aptamer so as to form the G tetrahedral structure. Therefore, the quantity of double-chain DNAs is reduced, and the fluorescence intensity of Sybr Green I is lowered. The fluorescence intensity is gradually decreased along with the increase of lead ion concentration. The fluorescence detection method has relat
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN107764785A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN107764785A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN107764785A3</originalsourceid><addsrcrecordid>eNrjZNB2yynNL0otTk7NS05VSEktSU0uyczPU8hNLcnIT1FIyy9SyElNTFEAihXzMLCmJeYUp_JCaW4GRTfXEGcP3dSC_PjU4oJEoCGpJfHOfoYG5uZmJuYWpo7GxKgBAGsIKYA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Fluorescence detection method for lead ions</title><source>esp@cenet</source><creator>XU HUI ; LYU JUBO ; ZHANG YAHUI</creator><creatorcontrib>XU HUI ; LYU JUBO ; ZHANG YAHUI</creatorcontrib><description>The invention discloses a fluorescence detection method for lead ions. By using the characteristic that a lead ion specificity DNA (Pb aptamer) (5'-GGGTGGGTGGGTGGGT-3') can be combined with lead ion specificity to form a G tetrahedral structure and the characteristic that a fluorescent dye Sybr Green I can be combined with a double-chain DNA so that the fluorescence intensity is greatly enhanced by compared with a free state, so that the lead ion specificity DNA (Pb aptamer) is combined with a partially complementary chain DNA (Pb aptamer-com), then the fluorescence intensity is greatly enhanced through the addition of the fluorescent dye Sybr Green I, and then lead ions are added to be combined with Pb aptamer so as to form the G tetrahedral structure. Therefore, the quantity of double-chain DNAs is reduced, and the fluorescence intensity of Sybr Green I is lowered. The fluorescence intensity is gradually decreased along with the increase of lead ion concentration. The fluorescence detection method has relat</description><language>chi ; eng</language><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; PHYSICS ; TESTING</subject><creationdate>2018</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20180306&amp;DB=EPODOC&amp;CC=CN&amp;NR=107764785A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20180306&amp;DB=EPODOC&amp;CC=CN&amp;NR=107764785A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>XU HUI</creatorcontrib><creatorcontrib>LYU JUBO</creatorcontrib><creatorcontrib>ZHANG YAHUI</creatorcontrib><title>Fluorescence detection method for lead ions</title><description>The invention discloses a fluorescence detection method for lead ions. By using the characteristic that a lead ion specificity DNA (Pb aptamer) (5'-GGGTGGGTGGGTGGGT-3') can be combined with lead ion specificity to form a G tetrahedral structure and the characteristic that a fluorescent dye Sybr Green I can be combined with a double-chain DNA so that the fluorescence intensity is greatly enhanced by compared with a free state, so that the lead ion specificity DNA (Pb aptamer) is combined with a partially complementary chain DNA (Pb aptamer-com), then the fluorescence intensity is greatly enhanced through the addition of the fluorescent dye Sybr Green I, and then lead ions are added to be combined with Pb aptamer so as to form the G tetrahedral structure. Therefore, the quantity of double-chain DNAs is reduced, and the fluorescence intensity of Sybr Green I is lowered. The fluorescence intensity is gradually decreased along with the increase of lead ion concentration. The fluorescence detection method has relat</description><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>PHYSICS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2018</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZNB2yynNL0otTk7NS05VSEktSU0uyczPU8hNLcnIT1FIyy9SyElNTFEAihXzMLCmJeYUp_JCaW4GRTfXEGcP3dSC_PjU4oJEoCGpJfHOfoYG5uZmJuYWpo7GxKgBAGsIKYA</recordid><startdate>20180306</startdate><enddate>20180306</enddate><creator>XU HUI</creator><creator>LYU JUBO</creator><creator>ZHANG YAHUI</creator><scope>EVB</scope></search><sort><creationdate>20180306</creationdate><title>Fluorescence detection method for lead ions</title><author>XU HUI ; LYU JUBO ; ZHANG YAHUI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN107764785A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2018</creationdate><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>PHYSICS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>XU HUI</creatorcontrib><creatorcontrib>LYU JUBO</creatorcontrib><creatorcontrib>ZHANG YAHUI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>XU HUI</au><au>LYU JUBO</au><au>ZHANG YAHUI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Fluorescence detection method for lead ions</title><date>2018-03-06</date><risdate>2018</risdate><abstract>The invention discloses a fluorescence detection method for lead ions. By using the characteristic that a lead ion specificity DNA (Pb aptamer) (5'-GGGTGGGTGGGTGGGT-3') can be combined with lead ion specificity to form a G tetrahedral structure and the characteristic that a fluorescent dye Sybr Green I can be combined with a double-chain DNA so that the fluorescence intensity is greatly enhanced by compared with a free state, so that the lead ion specificity DNA (Pb aptamer) is combined with a partially complementary chain DNA (Pb aptamer-com), then the fluorescence intensity is greatly enhanced through the addition of the fluorescent dye Sybr Green I, and then lead ions are added to be combined with Pb aptamer so as to form the G tetrahedral structure. Therefore, the quantity of double-chain DNAs is reduced, and the fluorescence intensity of Sybr Green I is lowered. The fluorescence intensity is gradually decreased along with the increase of lead ion concentration. The fluorescence detection method has relat</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language chi ; eng
recordid cdi_epo_espacenet_CN107764785A
source esp@cenet
subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Fluorescence detection method for lead ions
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T02%3A44%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=XU%20HUI&rft.date=2018-03-06&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN107764785A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true