Fluorescence detection method for lead ions

The invention discloses a fluorescence detection method for lead ions. By using the characteristic that a lead ion specificity DNA (Pb aptamer) (5'-GGGTGGGTGGGTGGGT-3') can be combined with lead ion specificity to form a G tetrahedral structure and the characteristic that a fluorescent dye...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	XU HUI, LYU JUBO, ZHANG YAHUI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING
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Beschreibung
Zusammenfassung:	The invention discloses a fluorescence detection method for lead ions. By using the characteristic that a lead ion specificity DNA (Pb aptamer) (5'-GGGTGGGTGGGTGGGT-3') can be combined with lead ion specificity to form a G tetrahedral structure and the characteristic that a fluorescent dye Sybr Green I can be combined with a double-chain DNA so that the fluorescence intensity is greatly enhanced by compared with a free state, so that the lead ion specificity DNA (Pb aptamer) is combined with a partially complementary chain DNA (Pb aptamer-com), then the fluorescence intensity is greatly enhanced through the addition of the fluorescent dye Sybr Green I, and then lead ions are added to be combined with Pb aptamer so as to form the G tetrahedral structure. Therefore, the quantity of double-chain DNAs is reduced, and the fluorescence intensity of Sybr Green I is lowered. The fluorescence intensity is gradually decreased along with the increase of lead ion concentration. The fluorescence detection method has relat