Preparation method of human plasmin

The invention relates to a preparation method of human plasmin. The preparation method comprises the steps of activating human plasminogen (Plg) by virtue of a plasminogen activator (PA) at room temperature so as to generate plasmin, adding a terminating agent to terminate reaction, and carrying out...

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Hauptverfasser: DENG ZHIJUN, YUE SHENGLAN, PENG YAN, CHEN KEJIN, HU YONG, WANG FEIFEI, LIN LIANZHEN, LI CESHENG, ZHOU YANXIANG, LI TAOJING, ZHOU ZHIJUN, XING YANTAO, LI JUAN, ZHAN QIAN, TAN MIN
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creator DENG ZHIJUN
YUE SHENGLAN
PENG YAN
CHEN KEJIN
HU YONG
WANG FEIFEI
LIN LIANZHEN
LI CESHENG
ZHOU YANXIANG
LI TAOJING
ZHOU ZHIJUN
XING YANTAO
LI JUAN
ZHAN QIAN
TAN MIN
description The invention relates to a preparation method of human plasmin. The preparation method comprises the steps of activating human plasminogen (Plg) by virtue of a plasminogen activator (PA) at room temperature so as to generate plasmin, adding a terminating agent to terminate reaction, and carrying out separation and purification, wherein the terminating agent is capable of inhibiting the degradationof plasmin. 本发明涉及种人纤维蛋白溶解酶的制备方法。本发明在常温条件下用纤维蛋白溶解酶原激活剂(Plasminogen Activator,PA)激活人纤维蛋白溶解酶原(Plasminogen,Plg)生成纤维蛋白溶解酶,激活后加入终止剂终止反应,同时该终止剂可抑制纤维蛋白溶解酶降解,然后进行分离纯化即可。
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The preparation method comprises the steps of activating human plasminogen (Plg) by virtue of a plasminogen activator (PA) at room temperature so as to generate plasmin, adding a terminating agent to terminate reaction, and carrying out separation and purification, wherein the terminating agent is capable of inhibiting the degradationof plasmin. 本发明涉及种人纤维蛋白溶解酶的制备方法。本发明在常温条件下用纤维蛋白溶解酶原激活剂(Plasminogen Activator,PA)激活人纤维蛋白溶解酶原(Plasminogen,Plg)生成纤维蛋白溶解酶,激活后加入终止剂终止反应,同时该终止剂可抑制纤维蛋白溶解酶降解,然后进行分离纯化即可。</abstract><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMES
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
SPIRITS
VINEGAR
WINE
title Preparation method of human plasmin
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