NF-kB dual-luciferase reporter cell for stably expressing swine TLR5 receptor genes and construction method

The invention belongs to the technical field of biology and in particular relates to an NF-kB dual-luciferase reporter cell for stably expressing swine TLR5 receptor genes and a construction method thereof. A cell line refers to HEK293-NF-kB cells in which the swine TLR5 receptor genes are transferred, and the HEK293-NF-kB cells refer to HEK293 cells in which NF-kB firefly luciferase and renilla luciferase are transferred. The invention provides the stable cell line used in detection for researching pTLR5 activated downstream NF-kB promoter report genes. The invention also provides a biological material for performing high-throughput screening on differentially expressed genes in stable cells in quiesced and activated states in an RNA-Seq technology, and lays a foundation and platform forfurther researching species specificity of TLR5. 本发明属于生物技术领域，具体涉及稳定表达猪源TLR5受体基因的NF-κB双荧光素酶报告细胞系及其构建方法。该细胞系是指转入了猪源TLR5受体基因的HEK293-NF-κB细胞，所述HEK293-NF-κB细胞是引入了NF-κB虫荧光素酶和内参海肾荧光素酶的HEK293细胞。本发明提供能够用于研究pTLR5活化激活下游NF-κB启动子报告基因检测稳定细