ELISA detection method of Fc[gamma]RIIIA receptor

The invention discloses an ELISA detection method of an Fc[gamma]RIIIA receptor, wherein the ELISA detection method comprises the steps: a coating step of coating an ELISA plate with the Fc[gamma]RIIIA, sealing, sample preparation, secondary antibody incubation, and substrate color development; the sample preparation step comprises the steps: after mixing evenly an anti-VEGF monoclonal antibody and an antigen, allowing to stand for an hour to form an immunocomplex. The detection method can detect the binding activity of the anti-VEGF monoclonal antibody and the Fc[gamma]RIIIA receptor in vitroquickly and directly, has the advantages of relatively low cost, convenient experimental operation, good repeatability, objective result judgment and high sensitivity, and also provides reference information for consistency evaluation of the anti-VEGF monoclonal antibody and study of clinical pharmacological efficacy and pharmacokinetics. 本发明要求保护种FcγRIIIA受体的ELISA检测方法，其包括在酶标板上包被FcγRIIIA受体的包被步骤、封闭、样品配制、二抗孵育、底物显色的步骤，且所述样品配制