Preparation and application of GP73 C-terminal antigen

The invention provides a GP73 C-terminal protein and an anti GP73 C-terminal monoclonal antibody prepared thereby; for solving the deficiency of lack of GP73 quantitative detection kits as a liver cirrhosis early diagnosis detection means in clinic currently, another objective of the invention is to provide a test kit having the advantages of being simple in operation, accurate, sensitive, stablein quality and capable of mass production and a determination method thereof, so as to observe the expression content of GP73 at a protein level, take effective integrated treatment schemes as soon aspossible and effectively prevent further malignancy of cirrhosis. 本发明提供种GP73 C端蛋白，由此制备抗GP73 C端的单克隆抗体，为了解决目前临床上缺乏GP73定量检测试剂盒作为肝硬化早期诊断检测手段的不足，本发明的另目的在于提供了种操作简便、准确灵敏、质量稳定、可大规模生产的检测试剂盒及其测定方法，以便从蛋白水平上观察GP73表达含量，能尽早采取有效的综合治疗方案，有效预防肝硬化的进步恶变。