Method for preparing protoplasts of Gracilaria bailinae

The invention discloses a method for preparing protoplasts of Gracilaria bailinae. The method produces an agarase crude solution through culture and fermentation of marine bacteria Marinomonas sp. YS-70, mixes it with cellulose and macerozyme and adds a buffer medium and an osmotic agent to form a protoplast separation solution and performs enzymolysis treatment on disinfected and chopped Gracilaria bailinae to release free protoplasts. The method adopts the agarase isolated from the protoplasts of Gracilaria bailinae, and the agarase contains a plurality of enzyme activities, has a large yield, can fully dissociate cell walls and has low cost and high efficiency. The macerozyme interacts with the cellulase and the agarase to work synergistically, so that the efficiency is high, the required time is short and it takes only 4 hours of dissociation to obtain a large number of protoplasts and the yield and the survival rate of the protoplasts can be improved. After dissociation, the activity of the protoplasts i