A preparing method of high-purity antivenene

A preparing method of high-purity antivenene is disclosed. The method includes materials and a preparing process. Digestion with pepsase, precipitation separation with n-caprylic acid and purificationwith ion chromatography are adopted. According to a technical scheme, a characteristic that the n-caprylic acid enables precipitation of impurity proteins except immune globulin (IgG) is utilized, precipitation is performed utilizing the n-caprylic acid to remove the impurity proteins, and then purification is performed through ion chromatography to increase purity of antivenene F(ab')2. Throughthe technical scheme, a problem that purity increase is difficult in the prior art and a disadvantage that allergy side effects on patients affected with snake venom are serious are solved, and the objective of increasing purification indexes of the antivenene F(ab')2 and reducing the allergy side effects on patients affected with snake venom is achieved. 本发明公开了种高纯度抗蛇毒血清制备方法，包括材料和制备工艺。本发明采用胃蛋白酶消化，正辛酸沉淀分离和离子层析提纯的制备工艺，利用正辛酸对