Enzyme linked immunosorbent assay kit for detecting human cystatin SN as well as preparation method and detection method

The invention relates to an enzyme linked immunosorbent assay kit for detecting human cystatin SN as well as a preparation method and a detection method. An enzyme linked immunosorbent assay kit of human cystatin SN (CST1) does not exist on the current market. The enzyme linked immunosorbent assay kit for detecting the human cystatin SN is characterized by comprising a coated elisa plate, an enzyme conjugate, a calibration product, a substrate, washing liquor and stop solution, wherein the elisa plate is a CST1 anti-body coated by polystyrene; the enzyme conjugate is a CST1 anti-body marked byHRP (horseradish peroxidase); the calibration product is a CST1 antigen; the substrate is a TMB (tetramethylbenzidine) color developing agent; the stop solution is 0.5mol/L sulfuric acid; the washingliquor is PB (polybutylene) buffer solution; 1,000mL of the washing liquor comprises 90g of NaCl, 29g of Na2HPO4. 12H2O, 2.97g of NaH2PO4. 2H2O and 10ml of tween 20. The enzyme linked immunosorbent assay kit for detecting the