Construction method of recombinant adenovirus containing HN gene and gD gene as well as recombinant adenovirus and application

The invention discloses a construction method of recombinant adenovirus containing an HN gene and a gD gene as well as the recombinant adenovirus and application. The construction method comprises thesteps of amplifying the HN gene and the gD gene respectively from an NDV F48E9 strain and an ILTV WG strain, enabling the obtained target genes to be cloned into a shuttle vector pDC315-MCS-EGFP, utilizing a liposome transfection method to enable recombinant adenovirus shuttle plasmid pDC315-HN-gD-EGFP and adenovirus cytoskeleton pBHGlox(delta)E1, 3Cre to be co-transfected into HEK293 cells, carrying out recombination and packaging to obtain the recombinant adenovirus pBH-HN-gD-EGFP containing the HN gene and the gD gene. The recombinant adenovirus is used for immunizing poultry so as to prevent Newcastle disease and infectious laryngotracheitis of the poultry; after a chicken is immunized by using the recombinant adenovirus, the antibody level of the chicken is obviously improved. 本发明公开了种HN基因和gD基因重组腺病毒的构建方法及重组腺病