Primer pairs for detecting three recessive genetic defect diseases of dairy cow and application thereof

The invention discloses primer pairs for detecting three recessive genetic defect diseases of a dairy cow and application thereof. A first pair of primers consists of oligonucleotide sequences which are as shown in SEQ NO.1 and SEQ NO.2 in a sequence table; a second pair of primers consists of oligonucleotide sequences which are as shown in SEQ NO.3 and SEQ NO.4 in the sequence table; a third pairof primers consists of oligonucleotide sequences which are as shown in SEQ NO.5 and SEQ NO.6 in the sequence table; the three pairs of the primers can be applied to preparation of a kit for diagnosing three recessive genetic defect diseases, namely, CVM, BLAD and DUMPS. The primer pairs for detecting the three recessive genetic defect diseases of the dairy cow and the application thereof have thebenefits that the gene mutation sites of the three recessive genetic defect diseases, namely, the CVM, the BLAD and the DUMPS of the dairy cow can be detected by applying a high-resolution fusion curve technology and the thre