Method for separating and purifying cycloartenol from coix lacryma-jobi stem leaves

The invention discloses a method for separating and purifying cycloartenol from coix lacryma-jobi stem leaves. The method includes the steps: crushing the dried coix lacryma-jobi stem leaves, adding ethyl alcohol for heating reflux extraction and concentrating an extract to obtain extract A; adding water in the extract A for dispersing, performing extraction with petroleum ether, and concentratingpart of the petroleum ether to obtain extract B; subjecting the extract B to silca column chromatography and to gradient elution with petroleum ether-ethyl acetate, collecting the components separated and purified, performing chromatography once more and elution with pure dichloromethane, subjecting the obtained components separated and purified to separation and purification via preparative highperformance high chromatography, collecting tR=34min components, and performing concentration and crystallization to obtain the cycloartenol. The cycloartenol is extracted from the waste coix lacryma-jobi stem leaves, environ