High-temperature-resisting saccharomyces cerevisiae strain and construction method thereof
The invention discloses a high-temperature-resisting saccharomyces cerevisiae strain and a construction method thereof, and belongs to the bioengineering technology field. SCH9 genes in Ras/cAMP/PKA signal channels are completely knocked out from a parent saccharomyces cerevisiae strain, and serine/...
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Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a high-temperature-resisting saccharomyces cerevisiae strain and a construction method thereof, and belongs to the bioengineering technology field. SCH9 genes in Ras/cAMP/PKA signal channels are completely knocked out from a parent saccharomyces cerevisiae strain, and serine/threonine protein kinase Sch9 in coding of the genes and catalytic subunits of PKA (protein kinase A) have high homology, so that knockout of the SCH9 genes can have influences on activity of the PKA, PKA activity is reduced, and pressure resistance of cells is improved. URA3 genes inherent in saccharomyces cerevisiae bodies serve as selection markers, and accordingly, residues of foreign genes are avoided. 48h cell viability, CO2 weightlessness and ethyl alcohol yield of the obtained high-temperature-resisting saccharomyces cerevisiae strain are improved, and the content of residual sugar is reduced; ethyl alcohol yield of a converter 2a of AY12a-SCH9 delta is increased by 16.4% as compared to the parent strain, t |
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