Indirect ELISA detection kit for small ruminant lentiviruses

The invention belongs to the field of biotechnology, and concretely relates to an indirect ELISA detection kit for small ruminant lentiviruses. The amino acid sequence of an oligopeptide P1 is represented by SEQ ID No.1, and the amino acid sequence of an oligopeptide P2 is represented by SEQ ID No.2. The two synthesized oligopeptides are used as antigens to coat an ELISA plate capable of specifically adsorbing the oligopeptides, and the coating concentration of the oligopeptide antigens, the concentration of detected serum and the dilution degree of an HRP-labeled rabbit anti-goat antibody areoptimized to establish an indirect ELISA method for detecting small ruminant lentivirus serum antibodies. 本发明属于生物技术领域，具体涉及种小反刍动物慢病毒间接ELISA检测试剂盒，寡肽P1和寡肽P2，如SEQ ID No.1何SEQ ID No.2所示的氨基酸序列。人工合成的两条寡肽作为抗原包被可特异性吸附寡肽的ELISA板，通过优化寡肽抗原包被浓度、被检血清浓度和HRP标记的兔抗山羊抗体的稀释度，建立了可以检测小反刍动物慢病毒血清抗体的间接ELISA方法。