Plasmids and method for obtaining viral particles

The invention relates to: a method for producing a negative single-strand RNA virus; a recombinant plasmid functional in animal cells, which consists of a pSS-URG skeleton; a method for producing viral particles; and a use for expressing RNA and proteins autogenous or exogenous to the virus. 本发明描述了病...

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Hauptverfasser: BELTRAN PAVEZ CAROLINA, SPENCER OSSA EUGENIO, TAMBLEY ZAMORANO CAROLINA, CORTEZ SAN MARTIN MARCELO, TORO ASCUY DANIELA
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creator BELTRAN PAVEZ CAROLINA
SPENCER OSSA EUGENIO
TAMBLEY ZAMORANO CAROLINA
CORTEZ SAN MARTIN MARCELO
TORO ASCUY DANIELA
description The invention relates to: a method for producing a negative single-strand RNA virus; a recombinant plasmid functional in animal cells, which consists of a pSS-URG skeleton; a method for producing viral particles; and a use for expressing RNA and proteins autogenous or exogenous to the virus. 本发明描述了病毒RNA表达质粒和基于所述质粒获得病毒颗粒的方法,其包括用能够表达核蛋白和RNA依赖性聚合酶RNA的表达载体或组表达载体转染动物细胞;并用具有编码重组RNA分子的核苷酸序列的表达载体或组表达载体转染动物细胞。
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Plasmids and method for obtaining viral particles
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