Plasmids and method for obtaining viral particles

The invention relates to: a method for producing a negative single-strand RNA virus; a recombinant plasmid functional in animal cells, which consists of a pSS-URG skeleton; a method for producing viral particles; and a use for expressing RNA and proteins autogenous or exogenous to the virus. 本发明描述了病...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	BELTRAN PAVEZ CAROLINA, SPENCER OSSA EUGENIO, TAMBLEY ZAMORANO CAROLINA, CORTEZ SAN MARTIN MARCELO, TORO ASCUY DANIELA
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to: a method for producing a negative single-strand RNA virus; a recombinant plasmid functional in animal cells, which consists of a pSS-URG skeleton; a method for producing viral particles; and a use for expressing RNA and proteins autogenous or exogenous to the virus. 本发明描述了病毒RNA表达质粒和基于所述质粒获得病毒颗粒的方法，其包括用能够表达核蛋白和RNA依赖性聚合酶RNA的表达载体或组表达载体转染动物细胞；并用具有编码重组RNA分子的核苷酸序列的表达载体或组表达载体转染动物细胞。