Ustilaginoidea virens spore real-time quantification loop-mediated isothermal amplication detection method and kit

The invention discloses an LAMP specific primer composition used for an ustilaginoidea virens spore q-LAMP rapid detection method. The LAMP specific primer composition consists of four primers such as upstream and downstream outer primers and upstream and downstream inner primers. The invention also discloses a loop-mediated isothermal amplication kit for quantitatively detecting ustilaginoidea virens spores. The loop-mediated isothermal amplication kit contains the LAMP specific primer composition, and also contains glycine betaine, 10 * SYBR Green I, 8 U/[mu]L Bst DNA polymerase and the like. The invention also correspondingly discloses a loop-mediated isothermal amplication method for quantitatively detecting ustilaginoidea virens spores. 本发明公开了种用于稻曲病菌孢子的q-LAMP快速检测方法的LAMP特异性引物组合物，该LAMP特异性引物组合物由上下游外引物、上下游内引物这四条引物组成。本发明还同时公开了种用于定量检测稻曲病菌孢子的环介导等温扩增试剂盒，其包含上述LAMP特异性引物组合物，还包含甜菜碱、10×SYBR Green I、8U/μL Bst DNA聚合酶等。本发明还相应公开了种用于定量检测稻曲病菌孢子的环介导等温扩增方法。