Method for inducing umbilical cord mesenchymal stem cell to differentiate to form insulin
The invention discloses a method for inducing an umbilical cord mesenchymal stem cell to differentiate to form insulin. The method comprises the following steps: separating and cultivating the umbilical cord mesenchymal stem cell in vitro; enabling a cell factor to induce the umbilical cord mesenchy...
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Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a method for inducing an umbilical cord mesenchymal stem cell to differentiate to form insulin. The method comprises the following steps: separating and cultivating the umbilical cord mesenchymal stem cell in vitro; enabling a cell factor to induce the umbilical cord mesenchymal stem cell to differentiate into an insulin cell: inoculating a third generation umbilical cord mesenchymal stem cell in a culture flask T25cm, adding an inducing liquid when the cell is fused by 80%-90%, and inducing the cell to obtain the insulin cell. The differentiating capability of the umbilical cord mesenchymal stem cell obtained by separating and cultivating umbilical cord blood is relatively strong, and the umbilical cord mesenchymal stem cell has a multi-directional differentiation potential, supports hematopoiesis and has an immunosuppression effect. Compared with donating bone marrow or drawing and mobilizing peripheral blood, a donator has no pain, the risk of infection is reduced, and the amplifica |
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