Library for performing high-throughput detection on circulating tumor DNA target genes, as well as detection method and application of library

The invention relates to the field of genomic high-throughput sequencing, in particular to a library for performing high-throughput detection on circulating tumor DNA target genes, as well as a detection method and application of the library. Circulating body fluid of a tumor patient is used as a bi...

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Bibliographische Detailangaben
Hauptverfasser:	CHEN ZHISHENG, LIU QIANG, ZHANG XIA, ZHANG YU, LI ZHIGUANG, CHEN JUN, LYU DEKANG, MA ZHAOKUI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the field of genomic high-throughput sequencing, in particular to a library for performing high-throughput detection on circulating tumor DNA target genes, as well as a detection method and application of the library. Circulating body fluid of a tumor patient is used as a biological sample, low-concentration free nucleic acid is extracted, the low-concentration free nucleic acid of the biological sample is used as a sample template, target gene exons of the sample template are amplified in a multiplex PCR form, then the tail ends of obtained amplicons are repaired and the 3' ends of the amplicons are connected with A basic groups, the modified amplicons are connected to Illumina sequencing adapters, and then library PCR is carried out by using an index-containing primer, and an amplified product is the library for detecting mutation diversity of the circulating tumor DNA target genes. The in-vitro circulating body fluid of the tumor patient is used as the biological sample for achievi