Bioconversion method of L-hydroxyproline

The present invention discloses a bioconversion method of L-hydroxyproline. According to the bioconversion method, a surfactant and an additive are added to establish a bioconversion system by using L-proline and alpha-ketoglutaric acid as substrates and using the whole cell of engineering bacteria containing proline-4-hydroxylase as a catalyst, and a conversion reaction is performed to obtain the conversion liquid containing L-hydroxyproline. According to the present invention, the bioconversion method has advantages of strong stereoselectivity, mild reaction conditions, easy operation, low cost, less pollution and less catalyst consumption, and can complete the reactions difficultly performed in chemical reactions, wherein the space time yield is more than 62.36 g/d/L, the yield is more than 90%, the crystal purity is more than 98%, and the optical purity is more than 99%. 本发明公开了种L-羟脯氨酸的生物转化方法，其特征在于，包括以下步骤：以L-脯氨酸、α-酮戊二酸为底物，以含脯氨酸-4-羟化酶的工程菌全细胞为催化剂，再添加表面活性剂和添加剂建立生物转化体系进行转化反应，得到含有L-羟脯氨酸的转化液。立体选择性强、反应条件温和、操作简便、成