Method for extracting total alkaloids from stephania sinica diels

The invention discloses a method for extracting total alkaloids from stephania sinica diels. The method includes smashing and sieving the stephania sinica diels, adding ethyl alcohol into the stephania sinica diels, carrying out reflux extraction and merging filter liquid; concentrating filter liqui...

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Bibliographische Detailangaben
Hauptverfasser: HUANG YULU, XIA XIAOYAN, LI JIN, HUANG SUOYI, YU QIAORONG, LI TINGSHU
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for extracting total alkaloids from stephania sinica diels. The method includes smashing and sieving the stephania sinica diels, adding ethyl alcohol into the stephania sinica diels, carrying out reflux extraction and merging filter liquid; concentrating filter liquid under reduced pressures to obtain stephania sinica diels crude extract, adding n-butyl alcohol into the stephania sinica diels crude extract, preparing sample injection solution from the stephania sinica diels crude extract and the n-butyl alcohol and regulating a pH (potential of hydrogen) value of the solution by the aid of dilute acid until the pH value of the solution reaches 2-3; delivering the sample injection solution to a simulation moving bed, and separating and purifying the sample injection solution to obtain eluent; concentrating the eluent under reduced pressures and drying the eluent under vacuum conditions to obtain the total alkaloids from the stephania sinica diels. Chromatographic columns of the