MODIFIED DNASE AND USES THEREOF

Modified DNase I protein in which one or more amino acids of a DNase I protein are modified non-cellularly, are provided. The modified DNase I protein exhibits a DNA hydrolytic activity in the presence of actin and an improved DNA hydrolytic activity compared to a homologous non-modified DNase I protein. Processes of preparing the modified DNase I protein and uses thereof in, for example, reducing a DNA content in sputum and/or in treating a disease or condition associated with excess extracellular DNA in a fluid, secretion or tissue of a subject, are also provided. 本发明提供种修饰的DNA酶I蛋白，其中DNA酶I蛋白的或多个氨基酸被非细胞地修饰。所述修饰的DNA酶I蛋白在肌动蛋白存在下展现DNA水解活性，以及相较于同源的未修饰的DNA酶I蛋白亦展现改良的DNA水解活性。本发明还提供多种制备修饰的DNA酶I蛋白的方法以及例如其在减少痰液中DNA含量和/或治疗与对象的流体、分泌物或组织中过量的细胞外DNA相关的疾病或病症的多种用途。