Diluent for long-term storage of Tibetan sheep sperm and preparation method of diluent

The invention discloses a diluent for long-term storage of Tibetan sheep sperm. The diluent is prepared from the following components: 90-110 ml of double-distilled water, 3-5 g of saccharides, 3-4 g of a buffering agent, 0.1-0.3 g of tris(hydroxymethyl)aminomethane, 3-7 ml of vitamin B12, 5-10 ml of an osmotic protective agent, 10-20 ml of egg yolk, 80,000-120,000 units of penicillin and 80,000-120,000 units of streptomycin. A preparation process of the diluent comprises the following steps: S1, preparation of a solution A; S2, preparation of a solution B; S3, preparation of ethylene glycol, S4, dilution of the sperm, S5, balancing; S6, freezing. The diluent in the formula has the effects of improving sperm motility, acrosome intact rate and cycle conception rate after cryopreservation, the cycle conception rate of efficient cultivation and artificial insemination of Tibetan sheep is increased, reproductive performance and economic benefit of the Tibetan sheep are remarkably improved, and the breed conservat