Recombinant canine long-acting interferon alpha, fusion protein for preparing long-acting interferon, and preparation method of fusion protein

The invention discloses recombinant canine long-acting interferon alpha, fusion protein for preparing the long-acting interferon, and a preparation method of the fusion protein. The fusion protein is formed by connecting canine interferon gamma and canine interferon alpha through flexible linker, and the fusion protein is blended with a lyoprotectant and then is freeze-dried so as to obtain the recombinant canine long-acting interferon alpha. The recombinant canine long-acting interferon alpha can remarkably prolong the half-life period of the canine interferon and prolongs the half-life period by 11 times or more compared with common canine interferon, has a broad-spectrum anti-virus effect, and improves the immune response of the canine. 本发明公开了种重组犬长效干扰素α及制备此长效干扰素的融合蛋白及其制备方法，所述融合蛋白由犬干扰素γ与犬干扰素α经柔性linker连接而形成，融合蛋白与冻干保护剂混配后经冷冻干燥即可得到重组犬长效干扰素α。所述重组犬长效干扰素α可显著提高犬干扰素的半衰期，较普通犬干扰素的半衰期提高11倍以上，并具有广谱抗病毒作用并能提高犬自身的免疫应答。