PCR detection kit for pneumocystis jiroveci
The invention provides a PCR detection kit for pneumocystis jiroveci. The PCR detection kit comprises a nucleic acid extracting solution, a PCR reaction solution, an enzyme mixed solution, a positive control and a negative control, wherein the PCR reaction solution contains a pair of amplification p...
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| creator | QIN WEI XIA CHENGQING |
| description | The invention provides a PCR detection kit for pneumocystis jiroveci. The PCR detection kit comprises a nucleic acid extracting solution, a PCR reaction solution, an enzyme mixed solution, a positive control and a negative control, wherein the PCR reaction solution contains a pair of amplification primers and an amplification specific probe which are designed aiming at a target sequence of the pneumocystis jiroveci, the amplification primers include a forward amplification primer and a reverse amplification primer, the forward amplification primer has a nucleotide sequence shown by SEQ ID NO.1, and the reverse amplification primer has a nucleotide sequence shown by SEQ ID NO.2; the amplification specific probe is formed by a circular ring sequence and two short handle sequences, and a terminal 5' of the amplification specific probe is marked with a fluorescence reporting group, and a terminal 3' of the amplification specific probe is marked with a fluorescence quenching group; and the sequence of SEQ ID NO.1 |
| format | Patent |
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The PCR detection kit comprises a nucleic acid extracting solution, a PCR reaction solution, an enzyme mixed solution, a positive control and a negative control, wherein the PCR reaction solution contains a pair of amplification primers and an amplification specific probe which are designed aiming at a target sequence of the pneumocystis jiroveci, the amplification primers include a forward amplification primer and a reverse amplification primer, the forward amplification primer has a nucleotide sequence shown by SEQ ID NO.1, and the reverse amplification primer has a nucleotide sequence shown by SEQ ID NO.2; the amplification specific probe is formed by a circular ring sequence and two short handle sequences, and a terminal 5' of the amplification specific probe is marked with a fluorescence reporting group, and a terminal 3' of the amplification specific probe is marked with a fluorescence quenching group; and the sequence of SEQ ID NO.1</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20171117&DB=EPODOC&CC=CN&NR=107354221A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20171117&DB=EPODOC&CC=CN&NR=107354221A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>QIN WEI</creatorcontrib><creatorcontrib>XIA CHENGQING</creatorcontrib><title>PCR detection kit for pneumocystis jiroveci</title><description>The invention provides a PCR detection kit for pneumocystis jiroveci. The PCR detection kit comprises a nucleic acid extracting solution, a PCR reaction solution, an enzyme mixed solution, a positive control and a negative control, wherein the PCR reaction solution contains a pair of amplification primers and an amplification specific probe which are designed aiming at a target sequence of the pneumocystis jiroveci, the amplification primers include a forward amplification primer and a reverse amplification primer, the forward amplification primer has a nucleotide sequence shown by SEQ ID NO.1, and the reverse amplification primer has a nucleotide sequence shown by SEQ ID NO.2; the amplification specific probe is formed by a circular ring sequence and two short handle sequences, and a terminal 5' of the amplification specific probe is marked with a fluorescence reporting group, and a terminal 3' of the amplification specific probe is marked with a fluorescence quenching group; and the sequence of SEQ ID NO.1</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZNAOcA5SSEktSU0uyczPU8jOLFFIyy9SKMhLLc3NT64sLsksVsjKLMovS03O5GFgTUvMKU7lhdLcDIpuriHOHrqpBfnxqcUFicmpeakl8c5-hgbmxqYmRkaGjsbEqAEAWWEpZQ</recordid><startdate>20171117</startdate><enddate>20171117</enddate><creator>QIN WEI</creator><creator>XIA CHENGQING</creator><scope>EVB</scope></search><sort><creationdate>20171117</creationdate><title>PCR detection kit for pneumocystis jiroveci</title><author>QIN WEI ; XIA CHENGQING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN107354221A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2017</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>QIN WEI</creatorcontrib><creatorcontrib>XIA CHENGQING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>QIN WEI</au><au>XIA CHENGQING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>PCR detection kit for pneumocystis jiroveci</title><date>2017-11-17</date><risdate>2017</risdate><abstract>The invention provides a PCR detection kit for pneumocystis jiroveci. The PCR detection kit comprises a nucleic acid extracting solution, a PCR reaction solution, an enzyme mixed solution, a positive control and a negative control, wherein the PCR reaction solution contains a pair of amplification primers and an amplification specific probe which are designed aiming at a target sequence of the pneumocystis jiroveci, the amplification primers include a forward amplification primer and a reverse amplification primer, the forward amplification primer has a nucleotide sequence shown by SEQ ID NO.1, and the reverse amplification primer has a nucleotide sequence shown by SEQ ID NO.2; the amplification specific probe is formed by a circular ring sequence and two short handle sequences, and a terminal 5' of the amplification specific probe is marked with a fluorescence reporting group, and a terminal 3' of the amplification specific probe is marked with a fluorescence quenching group; and the sequence of SEQ ID NO.1</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | PCR detection kit for pneumocystis jiroveci |
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