PCR detection kit for pneumocystis jiroveci
The invention provides a PCR detection kit for pneumocystis jiroveci. The PCR detection kit comprises a nucleic acid extracting solution, a PCR reaction solution, an enzyme mixed solution, a positive control and a negative control, wherein the PCR reaction solution contains a pair of amplification p...
Gespeichert in:
Hauptverfasser: | , |
---|---|
Format: | Patent |
Sprache: | chi ; eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The invention provides a PCR detection kit for pneumocystis jiroveci. The PCR detection kit comprises a nucleic acid extracting solution, a PCR reaction solution, an enzyme mixed solution, a positive control and a negative control, wherein the PCR reaction solution contains a pair of amplification primers and an amplification specific probe which are designed aiming at a target sequence of the pneumocystis jiroveci, the amplification primers include a forward amplification primer and a reverse amplification primer, the forward amplification primer has a nucleotide sequence shown by SEQ ID NO.1, and the reverse amplification primer has a nucleotide sequence shown by SEQ ID NO.2; the amplification specific probe is formed by a circular ring sequence and two short handle sequences, and a terminal 5' of the amplification specific probe is marked with a fluorescence reporting group, and a terminal 3' of the amplification specific probe is marked with a fluorescence quenching group; and the sequence of SEQ ID NO.1 |
---|