PCR detection kit for pneumocystis jiroveci

The invention provides a PCR detection kit for pneumocystis jiroveci. The PCR detection kit comprises a nucleic acid extracting solution, a PCR reaction solution, an enzyme mixed solution, a positive control and a negative control, wherein the PCR reaction solution contains a pair of amplification p...

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Hauptverfasser: QIN WEI, XIA CHENGQING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a PCR detection kit for pneumocystis jiroveci. The PCR detection kit comprises a nucleic acid extracting solution, a PCR reaction solution, an enzyme mixed solution, a positive control and a negative control, wherein the PCR reaction solution contains a pair of amplification primers and an amplification specific probe which are designed aiming at a target sequence of the pneumocystis jiroveci, the amplification primers include a forward amplification primer and a reverse amplification primer, the forward amplification primer has a nucleotide sequence shown by SEQ ID NO.1, and the reverse amplification primer has a nucleotide sequence shown by SEQ ID NO.2; the amplification specific probe is formed by a circular ring sequence and two short handle sequences, and a terminal 5' of the amplification specific probe is marked with a fluorescence reporting group, and a terminal 3' of the amplification specific probe is marked with a fluorescence quenching group; and the sequence of SEQ ID NO.1