Method for detecting content of cane sugar in vaccine and application of method

The invention discloses a method for detecting the content of cane sugar in a vaccine and application of the method. The method comprises the following steps: in a vaccine to be tested, performing capillary electrophoresis separation on ionized cane sugar under an alkali condition, and detecting ultraviolet absorption of a background electrolyte solution, thereby indirectly confirming the content of cane sugar, wherein the background electrolyte solution used in capillary electrophoresis is an alkali background electrolyte solution containing 2,6-dipicolinic acid. The capillary electrophoresis indirection ultraviolet detection method disclosed by the invention is rapid, simple and convenient, high in accuracy and relatively low in cost in detecting cane sugar, and has reference significances upon cane sugar content testing on other vaccine products with cane sugar as a protection agent. 本发明公开了种疫苗中蔗糖含量的检测方法及其应用，将待测疫苗中，在碱性条件下离子化后的蔗糖经毛细管电泳分离，毛细管电泳中的背景电解质溶液为含有2,6-吡啶二羧酸的碱性背景电解质溶液，通过检测背景电解质溶液的紫外吸收，间接确定蔗糖的含量。本发明提供的毛